The study will be carried out in a Dravet mouse model and the different phases of the project will be the following:

• The evaluation of the possible deregulation of the endocannabinoid system elements (receptors, enzymes for synthesis and degradation of the endocannabinoids, endocannabinoid ligands) in Dravet syndrome.
• The study of the epigenetic modulation of the endocannabinoid system in the disease
• The evaluation of cannabinoid compounds as a possible therapy
• The study of the endocannabinoid system as a possible diagnostic biomarker and in the treatment response

Regarding the economic needs of the project:

• € 15,000 for the maintenance of Dravet mice colonies and experimental animals.
• WT mice expressing SCN1a gene (C57BL6), nervous system SCN1a KO (SCN1a loxP / syn-cre) will be used
• € 45,000 for epigenetic studies (expression of miRNAs, DNA methylation etc.), immunocytochemistry, cytometry and western studies (primary and secondary antibodies), common reagents, drugs etc.
• € 5,000 for use of UCM Research Support Centers such as the cytometry center.
• € 5,000 for publication costs
• € 30,000 staff recruitment